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Induction of Ocular Surface Inflammation and Collection of Involved Tissues
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Tissue remodeling after ocular surface reconstruction with denuded amniotic membrane
Jing Jie1,2,3,4, Jie Yang1,2,3,5, Hui He1,2,3
1Eye Institute of Xiamen University, Xiamen, Fujian, China.
Scientific Reports
|April 25, 2018
Summary
Denuded amniotic membrane (dAM) promotes faster ocular surface healing and reduces scarring compared to intact amniotic membrane (iAM). This makes dAM a superior option for conjunctival and corneal limbal reconstruction.
Area of Science:
- Ophthalmology
- Regenerative Medicine
- Tissue Engineering
Background:
- Amniotic membrane (AM) is utilized for ocular surface diseases.
- Comparing intact AM (iAM) and denuded AM (dAM) is crucial for optimizing treatments.
Purpose of the Study:
- To compare epithelial wound healing and tissue remodeling between iAM and dAM grafts.
- To evaluate the impact of AM preparation on ocular surface reconstruction.
Main Methods:
- Ocular surface reconstruction in New Zealand rabbits using cryopreserved human iAM or dAM after partial limbal and conjunctival removal.
- In vivo and histological observations of epithelial ingrowth, stratification, goblet cell differentiation, collagen degradation, and inflammatory cell infiltration.
Main Results:
- Faster epithelial ingrowth observed on dAM compared to iAM.
- Enhanced epithelial stratification and increased goblet cell numbers on dAM at 2 weeks.
- Rapid degradation of Collagen VII in dAM, reduced macrophage and α-SMA positive cells, indicating less inflammation and scarring.
Conclusions:
- Denuded amniotic membrane (dAM) accelerates epithelial healing and goblet cell differentiation.
- dAM transplantation leads to reduced inflammation and scar formation in ocular surface reconstruction.
- dAM shows significant advantages over iAM for conjunctival and corneal limbal reconstruction.
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