Related Experiment Video

Updated: Feb 11, 2026

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
07:38

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

13.4K

Identification of Key Candidate Proteins and Pathways Associated with Temozolomide Resistance in Glioblastoma Based

Guo-Zhong Yi1,2, Wei Xiang3, Wen-Yan Feng4

  • 1Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.

Biomed Research International
|April 25, 2018
PubMed

Insights

This study identified key proteins and pathways linked to glioblastoma (GBM) resistance to temozolomide (TMZ). These findings may lead to new biomarkers for predicting or preventing TMZ resistance in GBM patients.

Area of Science:

  • Oncology
  • Proteomics
  • Bioinformatics

Background:

  • Temozolomide (TMZ) resistance is a major challenge in glioblastoma (GBM) treatment.
  • Identifying molecular mechanisms of resistance is crucial for improving patient outcomes.

Purpose of the Study:

  • To investigate proteins and pathways associated with TMZ resistance in GBM.
  • To identify potential biomarkers for predicting or preventing TMZ resistance.

Main Methods:

  • Proteomic analysis of U87 cells treated with TMZ.
  • Bioinformatic analyses including KEGG pathway and protein-protein interaction (PPI) network construction.
  • Validation of candidate proteins using The Cancer Genome Atlas (TCGA) dataset.

Main Results:

  • Identified 161 differentially expressed proteins (DEPs) in TMZ-treated U87 cells.
  • Upregulated DEPs were associated with actin cytoskeleton, focal adhesion, phagosome, and PI3K-AKT signaling pathways.
  • Nine key candidate proteins (DHX9, HNRNPR, RPL3, HNRNPA3, SF1, DDX5, EIF5B, BTF3, RPL8) involved in ribosome and spliceosome pathways were identified and associated with GBM patient prognosis.

Conclusions:

  • This study provides novel insights into the molecular underpinnings of TMZ resistance in GBM.
  • The identified proteins represent potential biomarkers for predicting or preventing TMZ resistance in glioblastoma.

More Related Videos

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

3.7K
Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules07:35

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules

Published on: December 16, 2021

2.8K

Related Concept Videos

Key Elements for Plant Nutrition02:35

Key Elements for Plant Nutrition

Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
24.4K
cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
8.6K
Key Techniques in Microbiology01:19

Key Techniques in Microbiology

Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
2.5K
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
8.9K
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.9K
C4 Pathway and CAM01:27

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
49.3K