Related Experiment Video
Updated: Feb 11, 2026

A Method For Production of Recombinant mCD1d Protein in Insect Cells.
Published on: December 10, 2007
Production of isotope-labeled proteins in insect cells for NMR
Bastian Franke1, Christian Opitz1, Shin Isogai1
1Focal Area Structural Biology and Biophysics, Biozentrum, University of Basel, 4056, Basel, Switzerland.
Abstract:
Baculovirus-infected insect cells have become a powerful tool to express recombinant proteins for structural and functional studies by NMR spectroscopy. This article provides an introduction into the insect cell/baculovirus expression system and its use for the production of recombinant isotope-labeled proteins. We discuss recent advances in inexpensive isotope-labeling methods using labeled algal or yeast extracts as the amino acid source and give examples of advanced NMR applications for proteins, which have become accessible by this eukaryotic expression host.
Related Concept Videos
Isotopes
An element's atomic mass, or weight,...
Osmoregulation in Insects
Elements: Chemical Symbols and Isotopes
Some symbols are derived from the common English name of the element; others are abbreviations of the name in another language — Latin, Greek or German. For example, the symbol for aluminum (common name)...
Isotopes and Radioisotopes
An isotope containing...
Mass Spectrometry: Isotope Effect
¹³C NMR: ¹H–¹³C Decoupling
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...

