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Updated: Feb 11, 2026

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
Published on: January 12, 2015
Isolation of Murine Adipose-Derived Stromal/Stem Cells Using an Explant Culture Method
Jie Li1,2,3, Hui Li3, Weidong Tian4
1Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, USA.
Abstract:
Adipose tissue provides valuable cell source for tissue engineering, regenerative medicine, and adipose tissue biology studies. The most widely used adipose-derived stromal/stem cells (ASCs) isolation protocol involves enzymatic digestion with collagenase. However, the yield of the method is poor even impossible to collect enough stromal vascular fraction (SVF) for expansion when the sample size is small, for instance only new born mice is available for cell culture. Here, we describe an efficient protocol for the isolation and expansion of ASCs using explant culture.
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