Related Experiment Video
Updated: Feb 11, 2026

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice
Published on: January 25, 2021
Isolation and Differentiation of Murine Primary Brown/Beige Preadipocytes
Hui Yu1, Margo Emont1,2, Heejin Jun1
1Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
Abstract:
Studies on thermogenic adipose cells (brown and beige fat) hold promise to treat obesity and its associated metabolic disorders due to the ability of these cells to dissipate energy in the form of heat. Although immortalized adipose cell lines have been widely used to investigate cell autonomous regulations, many physiological functions of thermogenic fat need to be studied with primary fat cells. Here, we present a detailed protocol of the isolation, culture, and differentiation of primary brown and beige preadipocytes from the stromal vascular fraction of murine interscapular and inguinal adipose depots.
Related Concept Videos
Primary and Secondary Growth in Roots and Shoots
Primary Active Transport
Implicit Differentiation
Logarithmic Differentiation
Cellular Differentiation
A zygote is a...
Differential Leveling

