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Related Concept Videos

Functions of Smooth Muscles01:23

Functions of Smooth Muscles

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Smooth muscles are an important type of muscle tissue that plays a vital role in the involuntary movements of internal organs. For example, they help regulate the movement of food through the gut and the flow of blood through the circulatory system.
Function of visceral smooth muscles
Visceral smooth muscle is found in the walls of all hollow organs, except the heart, and is a key player in the involuntary movements that drive the functioning of these internal organs. This tissue is arranged in...
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Smooth Muscle Contraction01:25

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Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
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Structure and Organization of Smooth Muscles01:13

Structure and Organization of Smooth Muscles

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Smooth muscle tissue is a type of muscle tissue that can be found lining various vital organs in the human body, including the lungs, blood vessels, digestive tract, and respiratory tract. This type of tissue is responsible for regulating the movements of these organs, playing crucial roles in the functioning of various systems, including the vascular, digestive, respiratory, and urinary systems.
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Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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Seedless Vascular Plants

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Seedless Vascular Plants Were the First Tall Plants on Earth
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Smooth Endoplasmic Reticulum01:21

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Smooth endoplasmic reticulum or smooth ER is a sub-organelle with specialized functions in animal cells and plant cells. It is often associated with the tubule morphology of the endoplasmic reticulum.
The ER provides optimal conditions for synthesizing steroid hormones and lipids, such as phospholipids and triglycerides. Traditionally, lipid metabolism was considered to be a smooth ER function. However, there is no direct evidence to prove that rough ER is completely excluded from lipid...
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Related Experiment Video

Updated: Feb 11, 2026

Isolation of Murine Coronary Vascular Smooth Muscle Cells
08:24

Isolation of Murine Coronary Vascular Smooth Muscle Cells

Published on: May 30, 2016

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Proteomic Profiling Exosomes from Vascular Smooth Muscle Cell.

Hong Qiu1, Songshan Shi1,2, Shunchun Wang1,2

  • 1Complex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA.

Proteomics. Clinical Applications
|April 25, 2018
PubMed
Summary

Vascular smooth muscle cell (VSMC)-derived exosomes contain 459 proteins involved in cell adhesion and platelet activation. These exosomes maintain vessel homeostasis rather than promoting angiogenesis, suggesting their role in vascular health.

Keywords:
angiogenesiscross-talkexosomesnanoLC-MS/MSvascular smooth muscle cells

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Related Experiment Videos

Last Updated: Feb 11, 2026

Isolation of Murine Coronary Vascular Smooth Muscle Cells
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Measuring Proliferation of Vascular Smooth Muscle Cells Using Click Chemistry
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Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation
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Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

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Area of Science:

  • Cell Biology
  • Vascular Biology
  • Proteomics

Background:

  • Vascular smooth muscle cells (VSMC) and endothelial cells (EC) engage in crucial bidirectional communication for vascular development and homeostasis.
  • Exosomes, small extracellular vesicles, are recognized as key mediators in this VSMC-EC crosstalk.
  • Understanding the protein cargo of exosomes is essential to elucidate their role in intercellular communication.

Purpose of the Study:

  • To comprehensively characterize the proteome of human VSMC-derived exosomes.
  • To investigate the functional implications of these exosomal proteins in VSMC-EC communication.
  • To assess the in vivo angiogenic potential of VSMC-derived exosomes.

Main Methods:

  • Proteomic analysis of human VSMC-derived exosomes using nano liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS).
  • Gene ontology analysis of identified exosomal proteins to determine their cellular components, molecular functions, and biological processes.
  • In vivo assessment of VSMC-derived exosomes for proangiogenic activity using an established angiogenesis assay.

Main Results:

  • Identification of 459 unique proteins within VSMC-derived exosomes.
  • Gene ontology analysis revealed significant enrichment in cell-cell adhesion and platelet activation/coagulation pathways.
  • VSMC-derived exosomes did not exhibit proangiogenic activity in vivo, indicating a role in maintaining vascular homeostasis.

Conclusions:

  • The proteomic characterization provides a systematic overview of proteins within VSMC-derived exosomes.
  • These findings highlight the potential regulatory roles of VSMC-derived exosomes in VSMC-EC communication.
  • Dysregulation of exosome-mediated functions may disrupt vascular homeostasis, contributing to the pathogenesis of vascular diseases.