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Updated: Feb 11, 2026
SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
Published on: March 3, 2017
Proteomic Analysis of Exosomes and Its Application in HIV-1 Infection
Collins Cheruiyot1, Zemplen Pataki1, Bharat Ramratnam1,2,3
1Department of Medicine, Division of Infectious Diseases, Laboratory of Retrovirology, Alpert Medical School of Brown University, Providence, RI, 02903, USA.
Abstract:
Exosomes are 30-100 nm extracellular vesicles secreted from late endosomes by various types of cells. Numerous studies have suggested that exosomes play significant roles in human immunodeficiency virus 1 (HIV-1) biogenesis. Proteomics coupled with exosome fractionation has been successfully used to identify various exosomal proteins and helped to uncover the interactions between exosomes and HIV-1. To inform the current progress in the intersection of exosome, proteomics, and HIV-1, this review is focused on: i) analyzing different exosome isolation, purification methods, and their implications in HIV-1 studies; ii) evaluating the roles of various proteomic techniques in defining exosomal contents; iii) discussing the research and clinical applications of proteomics and exosome in HIV-1 biology.
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