Germination of grass species in soil affected by crude oil contamination

Many grass species exist in the oil exploration areas of North Dakota. The objective of this study was to evaluate seed germination of 65 grass species affected by crude oil. Germination of all species was reduced by crude oil, ranging from 4.3 to 100%. Twenty-eight species were tolerant, 29 moderately tolerant, 6 moderately sensitive, and 2 sensitive. Based on the tolerance levels, the following were used to further test the dose response to crude oil: strong creeping red fescue (Festuca rubra L. ssp. rubra), perennial ryegrass (Lolium perenne L.), orchardgrass (Dactylis glomerata L.), buffalograss [Buchloe dactyloides (Nutt.) Engelm.], little bluestem [Schizachyrium scoparium (Michx.) Nash], witchgrass (Panicum capillare L.), sand dropseed [Sporobolus cryptandrus (Torr.) Gray], Johnsongrass [Sorghum halepense (L.) Pers.], and smooth crabgrass [Digitaria ischaemum (Schreb.) Schreb. ex Muhl.]. The EC₅₀ of germination and biomass was effective in ranking the 9 species. Buffalograss, sand dropseed, and orchardgrass were ranked as the most tolerant species with EC₅₀ values of 0.1, > highest concentration tested, 0.05 m³ m^-3 (P < 0.05), respectively. Smooth crabgrass and little bluestem were ranked as most sensitive with EC₅₀ values of 0.03 and 0.04 m³ m^-3 (P < 0.05), respectively. Buffalograss showed the lowest germination (10.4%) and biomass reduction (25%) (P < 0.05).