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Turning lambda Cro into a transcriptional activator.

F D Bushman1, M Ptashne

  • 1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.

Cell
|July 15, 1988
PubMed
Summary
This summary is machine-generated.

Lambda repressor activates transcription by interacting with RNA polymerase. Researchers transferred this "activating patch" to Cro protein, creating a new transcriptional activator, demonstrating its sufficiency for activation.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Lambda repressor binding to DNA stimulates transcription via interaction with RNA polymerase.
  • Mutational analysis identified an acidic patch on lambda repressor crucial for transcriptional activation.

Purpose of the Study:

  • To determine if the identified "activating patch" of lambda repressor is sufficient for transcriptional activation.
  • To investigate the role of this activating patch in other proteins and phages.

Main Methods:

  • Isolation of lambda repressor mutants impaired in transcriptional activation.
  • Transferring the "activating patch" from lambda repressor to lambda Cro protein.
  • Analyzing the transcriptional activity of the modified Cro protein.

Main Results:

  • The "activating patch" of lambda repressor was found to be sufficient for transcriptional activation in a new sequence context.
  • Modified lambda Cro protein, containing the repressor's activating patch, exhibited transcriptional activator properties.
  • Evidence suggests phage 434 repressor utilizes a similar activating patch for transcription.

Conclusions:

  • The acidic "activating patch" on lambda repressor is a key functional domain for transcriptional activation.
  • This activating patch can confer transcriptional activation capabilities to other proteins.
  • Conserved mechanisms of transcriptional activation involving similar activating patches may exist across different phage repressors.