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The Complete and Updated "Rotifer Polyculture Method" for Rearing First Feeding Zebrafish
Published on: January 17, 2016
Dried Rotifer Sheet: A Novel Live Feed for Rearing First-Feeding Larvae
Hiroko Nakayama1, Kan Katayama2, Yukimasa Onabe3
11 Graduate School of Regional Innovation Studies, Mie University , Tsu, Japan .
Abstract:
Rotifers are now becoming a standard live feed for first-feeding larvae, because of their easy rearing protocol and improved growth and survival rate, compared with other fish feed, paramecia, and processed powder fish feeds. In a group of rotifers, bdelloid rotifer, a freshwater plankton, exerts cryptobiosis (an ametabolic state of life) in response to desiccation and survived for a long time. Here, we created "dried rotifer sheet" for rearing larvae in their early developmental stage. More than 80% rotifers could survive after 18-month desiccation in the filter paper. Larvae fed with the revived rotifer from dried rotifer sheet exhibited the higher survival rate with less pollution of their breeding water, compared with fine-powdered processed feeds or live paramecia. Our new feeding method is suitable for rearing larvae, and enables screening experiments with first-feeding larvae.
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