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Determination of the Glycogen Content in Cyanobacteria
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Engineering isoprene synthesis in cyanobacteria.

Julie E Chaves1, Anastasios Melis1

  • 1Plant and Microbial Biology, University of California, Berkeley, CA, USA.

FEBS Letters
|April 25, 2018
PubMed
Summary

Renewable isoprene (Isp) production is explored using engineered microbes. This review covers metabolic engineering strategies for sustainable Isp hydrocarbon fuel and feedstock generation.

Keywords:
Synechocystisbioenergydimethylallyl diphosphateisopentenyl diphosphateisoprenemetabolic engineeringsynthetic biologyterpenoids

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Area of Science:

  • Biotechnology and Synthetic Biology
  • Sustainable Chemistry

Background:

  • Isoprene (Isp) is a valuable hydrocarbon naturally produced by plants.
  • Renewable production of Isp is sought for fuel and chemical feedstock applications.
  • Current interest focuses on engineering microorganisms for continuous Isp synthesis.

Purpose of the Study:

  • To review the potential of microbial production of isoprene.
  • To examine metabolic engineering strategies for renewable Isp hydrocarbon synthesis.
  • To assess progress and challenges in this emerging research area.

Main Methods:

  • Review of scientific literature on isoprene biosynthesis and metabolic engineering.
  • Analysis of heterologous expression of Isp synthesis pathways in microorganisms.
  • Evaluation of current advancements and identified barriers.

Main Results:

  • Metabolic engineering enables the heterologous expression of Isp synthesis pathways.
  • Photosynthetic microorganisms are being developed for continuous renewable Isp production.
  • Significant progress has been made, but challenges remain in optimizing yield and scalability.

Conclusions:

  • Microbial production of isoprene holds promise for sustainable fuel and chemical feedstock.
  • Further metabolic engineering and process optimization are crucial for industrial viability.
  • This field represents a novel approach to renewable hydrocarbon generation.