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RNA-Seq analysis of interferon inducible p204-mediated network in anti-tumor immunity

Jinlong Jian1,2, Wei Wei1, Guowei Yin1

  • 1Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan, 250100, China.

Scientific Reports
|April 26, 2018
PubMed

Insights

The p204 protein is crucial for anti-tumor immunity. Its absence in mice significantly alters immune gene expression, particularly in response to lung cancer, revealing its role in the immune network.

Area of Science:

  • Immunology
  • Molecular Biology
  • Oncology

Background:

  • The tumor suppressor role of p204 (murine) and IFI16 (human) is established in vitro, but in vivo mechanisms are unclear.
  • Interferon-inducible p200 family proteins are implicated in cellular processes, including tumor suppression.

Purpose of the Study:

  • To investigate the in vivo role of p204 in anti-tumor immune response using a Lewis Lung carcinoma (LLC) model.
  • To compare the transcriptome of p204 null (KO) and wild-type (WT) mice to identify molecular events associated with p204 deficiency.

Main Methods:

  • Lewis Lung carcinoma (LLC) model induced in p204 KO and WT mice.
  • High-throughput RNA-sequencing of spleen transcriptome.
  • Differential gene expression analysis and Gene Ontology (GO) classification.

Main Results:

  • Minimal gene expression changes in untreated p204 KO vs. WT mice (11 genes).
  • Significant alterations in gene expression in tumor-bearing p204 KO vs. WT mice (393 genes).
  • Upregulation of cytokines, cytokine receptors, chemoattractants, and adhesion molecules in p204 KO mice with tumors.

Conclusions:

  • p204 plays a significant role in regulating the anti-tumor immune response in vivo.
  • Loss of p204 impacts immune cell signaling pathways, including those involving cytokines and cell adhesion.
  • This study provides a foundation for understanding p204-mediated gene expression in cancer immunity.

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