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Isolating the direct effects of adverse developmental conditions on in vivo cardiovascular function at adulthood: the

K L Skeffington1, C Beck1, N Itani1

  • 1Department of Physiology, Development & Neuroscience,University of Cambridge,Cambridge,UK.

Journal of Developmental Origins of Health and Disease
|April 26, 2018
PubMed

Insights

Researchers developed new surgical methods for adult chickens, enabling direct study of fetal programming and disease development. This avian model research isolates environmental impacts on fetal physiology for future disease consequence analysis.

Area of Science:

  • Physiology
  • Developmental Biology
  • Animal Models

Background:

  • In utero exposure to adverse environmental conditions can lead to later-life disease.
  • Avian models offer a unique advantage for studying fetal programming by isolating direct environmental effects.
  • Existing research is limited by a lack of established surgical protocols for adult avian models.

Purpose of the Study:

  • To establish reliable surgical protocols for adult avian models.
  • To enable direct investigation of fetal programming and cardiometabolic function in chickens.
  • To facilitate the study of long-term disease consequences from direct fetal environmental insults.

Main Methods:

  • Surgical instrumentation of seven adult Bovan Brown chickens with femoral arterial and venous catheters and a flow probe.
  • Evaluation of anesthetic agents (isoflurane and lidocaine) for their cardiovascular effects.
  • Description of optimized anesthesia, monitoring, surgical approach, postoperative care, and experimental procedures.

Main Results:

  • Established successful surgical protocols for adult chickens.
  • Observed depressive cardiovascular effects of isoflurane and lidocaine.
  • Demonstrated rapid recovery from surgery with no significant blood gas disturbances.
  • Successfully performed basal in vivo cardiovascular studies after 5 days of recovery.

Conclusions:

  • Developed and validated surgical techniques for adult avian cardiometabolic research.
  • The established protocols allow for detailed investigation of fetal programming in isolation from maternal effects.
  • This model is crucial for understanding the long-term health consequences of early-life environmental exposures.

Keywords:
in vivo studiesavian surgerycardiovascular diseaseprogramming

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