Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

2.0K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.0K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

2.1K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.1K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.9K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.9K
Epigenetic Regulation01:37

Epigenetic Regulation

3.3K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.3K
Epigenetic Regulation01:46

Epigenetic Regulation

31.9K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
31.9K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

24.1K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coordinated Temporal Dynamics of Glucocorticoid Receptor Binding and Chromatin Landscape Drive Transcriptional Regulation.

bioRxiv : the preprint server for biology·2026
Same author

QuantiTrack: A unified software to study protein dynamics in living cells.

bioRxiv : the preprint server for biology·2026
Same author

Corrigendum to "The impact of mineralocorticoid and glucocorticoid receptor interaction on corticosteroid transcriptional outcomes" [Molecul. Cell. Endocrinol. 594 (2024) 112389].

Molecular and cellular endocrinology·2025
Same author

The multimerization pathway of the glucocorticoid receptor.

Nucleic acids research·2025
Same author

Bile acids target an exposed cavity in the glucocorticoid receptor modulating receptor self-assembly, chromatin binding and transcriptional activity.

bioRxiv : the preprint server for biology·2025
Same author

Androgen receptor-mediated assisted loading of the glucocorticoid receptor modulates transcriptional responses in prostate cancer cells.

Genome research·2025

Related Experiment Video

Updated: Nov 7, 2025

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets
07:08

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets

Published on: February 2, 2024

1.1K

Chromatin reprogramming in breast cancer.

Erin E Swinstead1, Ville Paakinaho1,2, Gordon L Hager3

  • 1Laboratory of Receptor Biology and Gene ExpressionNational Cancer Institute, NIH, Bethesda, Maryland, USA.

Endocrine-Related Cancer
|April 26, 2018
PubMed
Summary

This review explores how transcription factors (TFs) and steroid receptors (SRs) reprogram chromatin and enhancer elements in breast cancer. Understanding these dynamics is key to unraveling breast cancer proliferation mechanisms.

Keywords:
ChIP-seqbreast cancerchromatinchromatin dynamicsenhancerestrogen receptorsingle moleculesteroid receptor

More Related Videos

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
10:41

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

10.6K
Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer
10:36

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer

Published on: March 17, 2016

10.7K

Related Experiment Videos

Last Updated: Nov 7, 2025

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets
07:08

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets

Published on: February 2, 2024

1.1K
An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
10:41

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

10.6K
Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer
10:36

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer

Published on: March 17, 2016

10.7K

Area of Science:

  • Molecular Biology
  • Genomics
  • Cancer Research

Background:

  • Chromatin landscape reprogramming is crucial for breast cancer transcriptional response.
  • Sex hormones like estrogens and progesterone significantly impact breast cancer proliferation.
  • The intricate signaling network of chromatin, enhancers, and transcription factors (TFs) in breast cancer remains mechanistically unclear.

Purpose of the Study:

  • To review the current understanding of the dynamic interplay between TFs, chromatin, and enhancer reprogramming in breast cancer.
  • To characterize the distinct modes of TF action in regulating enhancer activity, focusing on steroid receptors (SRs).
  • To elucidate how SRs target enhancer regions to reprogram chromatin in breast cancer cells.

Main Methods:

  • Review of existing literature on TF-chromatin interactions and enhancer function.
  • Discussion of genome-wide techniques for studying enhancer activity.
  • Highlighting advances in live-cell imaging for real-time analysis of SRs and chromatin dynamics.

Main Results:

  • TFs dynamically interact with chromatin to reprogram enhancer elements.
  • Steroid receptors (SRs) employ specific modes of action to target enhancers and modify chromatin in breast cancer.
  • Current techniques allow for genome-wide assessment of enhancer function and real-time single-cell studies.

Conclusions:

  • Further research into TF and SR mechanisms is essential for understanding breast cancer progression.
  • Advanced imaging techniques offer new avenues for studying chromatin dynamics in breast cancer.
  • Elucidating these molecular mechanisms can lead to novel therapeutic strategies for breast cancer.