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Updated: Feb 11, 2026

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
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Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Published on: March 20, 2020

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miR‑30a inhibits epithelial‑mesenchymal transition and metastasis in triple‑negative breast cancer by targeting ROR1

Xin Wang1, Huisi Qiu2, Ruiming Tang2

  • 1Department of Radiation Oncology, Nan Fang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

Oncology Reports
|April 26, 2018
PubMed

Insights

MicroRNA-30a (miR-30a) is decreased in triple-negative breast cancer (TNBC), suppressing its growth and metastasis. Targeting miR-30a offers a potential therapeutic strategy for aggressive TNBC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Triple-negative breast cancer (TNBC) is an aggressive subtype lacking targeted therapies.
  • MicroRNA-30a (miR-30a) expression is significantly reduced in TNBC.
  • Low miR-30a correlates with high histological grade and lymph node metastasis in TNBC patients.

Purpose of the Study:

  • To investigate the role of miR-30a in TNBC progression and metastasis.
  • To identify the molecular mechanisms underlying miR-30a's function in TNBC.
  • To evaluate miR-30a as a potential therapeutic target for TNBC.

Main Methods:

  • Quantitative analysis of miR-30a expression in TNBC tissues.
  • In vitro studies assessing the effects of miR-30a on epithelial-mesenchymal transition (EMT), invasion, and migration.
  • In vivo studies evaluating tumor growth and metastasis inhibition.
  • Luciferase assays to confirm the direct targeting of ROR1 by miR-30a.

Main Results:

  • miR-30a suppresses TNBC cell EMT by increasing E-cadherin and decreasing N-cadherin and vimentin.
  • Overexpression of miR-30a inhibits TNBC cell invasion, migration, tumor growth, and metastasis in vivo.
  • RTK-like orphan receptor 1 (ROR1) is identified as a direct target of miR-30a.
  • ROR1 overexpression reverses the inhibitory effects of miR-30a on TNBC cell migration and invasion.

Conclusions:

  • miR-30a acts as a tumor-metastasis suppressor miRNA in TNBC by directly targeting ROR1.
  • Restoring miR-30a levels could be a novel therapeutic strategy for TNBC.
  • miR-30a represents a promising therapeutic target for aggressive TNBC.

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