Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.6K
Uniform Depth Channel Flow01:27

Uniform Depth Channel Flow

612
Uniform depth channel flow keeps fluid depth consistent along channels such as irrigation canals. In natural channels, such as rivers, approximate uniform flow is often assumed. This condition occurs when the channel’s bottom slope matches the energy slope, balancing potential energy lost from gravity with head loss due to shear stress. This balance prevents depth changes along the channel length, resulting in a steady, uniform flow.Uniform flow in open channels with a constant cross-section...
612
Depth Perception and Spatial Vision01:15

Depth Perception and Spatial Vision

2.1K
Depth perception is the ability to perceive objects three-dimensionally. It relies on two types of cues: binocular and monocular. Binocular cues depend on the combination of images from both eyes and how the eyes work together. Since the eyes are in slightly different positions, each eye captures a slightly different image. This disparity between images, known as binocular disparity, helps the brain interpret depth. When the brain compares these images, it determines the distance to an object.
2.1K
Uniform Depth Channel Flow: Problem Solving01:18

Uniform Depth Channel Flow: Problem Solving

519
To calculate the flow rate for a trapezoidal channel, first, identify the bottom width, side slope, and flow depth of the channel. The cross-sectional area (A) corresponding to the depth of flow (y), channel bottom width (B), and side slope (θ) is determined by:Next, calculate the wetted perimeter, which includes the bottom width and the sloped side lengths in contact with the water. Using the values of the cross-sectional area and the wetted perimeter, determine the hydraulic radius by...
519
Gravitational Potential Energy for Extended Objects01:07

Gravitational Potential Energy for Extended Objects

2.0K
Consider a system comprising several point masses. The coordinates of the center of mass for this system can be expressed as the summation of the product of each mass and its position vector divided by the total mass:
2.0K
Assessment of Ventilation II: Respiratory Depth and Rhythm01:29

Assessment of Ventilation II: Respiratory Depth and Rhythm

2.6K
Respiratory Depth
Respiratory depth measures the volume of air inhaled or exhaled during a breath. It can vary from shallow to deep and typically remains consistent when a person is at rest or asleep. Occasionally, individuals will automatically inhale deeply, known as sighing, which inflates the lungs with more air than normal breathing.
To assess respiratory depth, observe the degree of chest excursion or movement:
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multifocal Pixel/Photon-Reassignment FLIM (MPPR-FLIM): A Super-Resolution Analytical Tool for Characterizing Subcellular Fluorescence Lifetime Heterogeneity via TCSPC.

Analytical chemistry·2026
Same author

De Novo Design of Near-Infrared Fluorescence-Activating Proteins.

Journal of the American Chemical Society·2026
Same author

High-throughput, organ-scale 3D tubule tracking using TubuleMAP.

bioRxiv : the preprint server for biology·2026
Same author

Paralleled needle-like-beam image scanning microscopy for fast volumetric super-resolution imaging.

Optics express·2026
Same author

Descattering and image restoration with a transformer-based neural network in deep tissue imaging.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Zero-shot learning for denoising and super-resolution in multifocal structured illumination microscopy.

Optics express·2025
Same journal

Anisotropic unbinding and location-dependent hovering of a kinesin motor head over microtubule.

Biophysical journal·2026
Same journal

Kinesin-5/Cut7 C-terminal tail phosphorylation influence on motor regulation through multi-scale molecular modeling.

Biophysical journal·2026
Same journal

Dynamic conformations of fluorophores on self-labeling protein tags.

Biophysical journal·2026
Same journal

Different actions of RyR2 open and closed channel block explained by a multiscale Ca<sup>2+</sup> release model.

Biophysical journal·2026
Same journal

Membrane Environment Sets the Functional pK<sub>a</sub> of Ionizable Lipids.

Biophysical journal·2026
Same journal

Distinguishable spreading dynamics in microbial communities.

Biophysical journal·2026
See all related articles

Related Experiment Video

Updated: Feb 11, 2026

Test Samples for Optimizing STORM Super-Resolution Microscopy
16:52

Test Samples for Optimizing STORM Super-Resolution Microscopy

Published on: September 6, 2013

31.7K

Extended-Depth 3D Super-Resolution Imaging Using Probe-Refresh STORM.

Danying Lin1, Lauren A Gagnon2, Marco D Howard2

  • 1Department of Chemistry, University of Washington, Seattle, Washington; Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, People's Republic of China.

Biophysical Journal
|April 26, 2018
PubMed
Summary
This summary is machine-generated.

Probe-refresh STORM (prSTORM) enables super-resolution microscopy of thicker samples by continuously replacing bleached fluorescent probes. This advanced imaging technique overcomes depth limitations in 3D microscopy.

More Related Videos

Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus
10:09

Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

Published on: November 6, 2018

6.8K
Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

5.3K

Related Experiment Videos

Last Updated: Feb 11, 2026

Test Samples for Optimizing STORM Super-Resolution Microscopy
16:52

Test Samples for Optimizing STORM Super-Resolution Microscopy

Published on: September 6, 2013

31.7K
Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus
10:09

Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

Published on: November 6, 2018

6.8K
Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

5.3K

Area of Science:

  • Biophysics
  • Microscopy
  • Molecular Imaging

Background:

  • Super-resolution fluorescence microscopy, including STORM, is typically limited to thin biological sections due to fluorophore photobleaching.
  • Imaging deeper sections compromises image quality by reducing localization density per plane.
  • Existing methods struggle to achieve high-resolution 3D imaging over extended depths.

Purpose of the Study:

  • To develop a novel imaging method to overcome depth limitations in 3D super-resolution microscopy.
  • To enable high-density localization imaging in extended depth 3D samples without sacrificing resolution.
  • To adapt the method for high-quality, 3D, multichannel imaging in fixed biological samples.

Main Methods:

  • Developed probe-refresh STORM (prSTORM), a method for replacing bleached fluorophores with fresh ones during imaging.
  • Utilized DNA-conjugated antibodies for sample staining and fluorescently-labeled DNA-reporter oligonucleotides for readout.
  • Enabled sequential replacement of reporter oligonucleotides in successive imaging rounds.

Main Results:

  • prSTORM successfully acquired 3D super-resolution images over extended depths in fixed samples.
  • The method maintained high localization density at each imaging plane, overcoming previous limitations.
  • Demonstrated adaptability for high-quality, 3D, multichannel imaging with extended depth.

Conclusions:

  • Probe-refresh STORM significantly advances 3D super-resolution microscopy by enabling imaging of thicker specimens.
  • This technique overcomes photobleaching limitations, allowing for improved image quality and depth penetration.
  • prSTORM offers a powerful tool for high-resolution 3D imaging in various biological applications.