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CircRNA-Cdr1as Exerts Anti-Oncogenic Functions in Bladder Cancer by Sponging MicroRNA-135a

Peng Li1,2, Xiao Yang1, Wenbo Yuan1

  • 1Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|April 26, 2018
PubMed
Abstract

Insights

Circular RNAs (CircRNAs) like Cdr1as are downregulated in bladder cancer, inhibiting tumor growth by sponging microRNA-135a. This finding reveals Cdr1as as a potential therapeutic target for bladder cancer treatment.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • Circular RNAs (CircRNAs) are implicated in various cancers.
  • The specific role and mechanism of Cdr1as in bladder cancer tumorigenesis were previously unknown.

Purpose of the Study:

  • To investigate the role of Cdr1as in bladder cancer development.
  • To elucidate the underlying molecular mechanisms of Cdr1as in bladder cancer.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for expression analysis.
  • In vitro and in vivo functional assays to assess Cdr1as effects on bladder cancer cell behavior.
  • Bioinformatic analysis, Ago2 immunoprecipitation, RNA pull-down, and FISH to confirm Cdr1as-miRNA interactions.

Main Results:

  • Cdr1as expression was significantly reduced in bladder cancer tissues.
  • Overexpressing Cdr1as suppressed bladder cancer cell proliferation, invasion, and migration.
  • Cdr1as directly interacted with and inhibited miR-135a activity, acting as a miRNA sponge.

Conclusions:

  • Cdr1as functions as an anti-oncogene in bladder cancer by downregulating miR-135a.
  • Cdr1as's tumor-suppressive role is mediated through sponging multiple microRNAs, notably miR-135a.
Keywords:
Bladder cancerCdr1asCircRNAMiR-135aP21

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