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Updated: Feb 11, 2026

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis
Published on: May 10, 2020
[Effects of nitrogen and irrigation water application on yield, water and nitrogen utilization and soil nitrate
Zhuan Yun Si1, Yang Gao1, Xiao Jun Shen1
1Ministry of Agriculture Key Laboratory of Crop Water Use and Its Regulation, Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences, Xinxiang 453002, Henan, China.
Abstract:
A field experiment was carried out to study the effects of nitrogen and irrigation water application on growth, yield, and water and nitrogen use efficiency of summer cotton, and to develop the optimal water and nitrogen management model for suitable yield and less nitrogen loss in summer cotton field in the Huang-Huai region. Two experimental factors were arranged in a split plot design. The main plots were used for arranging nitrogen factor which consisted of five nitrogen fertilizer le-vels(0, 60, 120, 180, 240 kg·hm-2, referred as N0, N1, N2, N3, N4), and the subplots for irrigation factor which consisted of three irrigation quota levels (30, 22.5, 15 mm, referred as I1, I2, I3). There were 15 treatments with three replications. Water was applied with drip irrigation system. Experimental results showed that both irrigation and nitrogen fertilization promoted cotton growth and yield obviously, but nitrogen fertilizer showed more important effects than irrigation and was the main factor of regulating growth and yield of summer cotton in the experimental region. With the increase of nitrogen fertilization rate and irrigation amount, the dry mater accumulation of reproductive organs, the above-ground biomass at the flowering-bolling stage and seed cotton yield increased gradually, reached peak values at nitrogen fertilization rate of 180 kg·hm-2 and decreased slowly with the nitrogen fertilization rate further increased. The maximum yield of 4016 kg·hm-2 was observed in the treatment of N3I1. Increasing nitrogen fertilizer amount would improve significantly total N absorption of shoots and N content of stem and leaf, but decrease nitrogen partial factor productivity. The maximum irrigation-water use efficiency of 5.40 kg·m-3 and field water use efficiency of 1.24 kg·m-3 were found in the treatments of N3I3 and N3I1, respectively. With increasing nitrogen fertilization amount, soil NO3--N content increased and the main soil NO3--N accumulation layer moved downward. By comprehensively considering above-ground biomass, seed cotton yield, water and nitrogen uptake and utilization, and soil NO3--N accumulation in the soil profile, the treatment N3I1 could be recommended as the optimal water and nitrogen application pattern for summer cotton production in the experimental region.
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