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Related Experiment Video

Updated: Feb 11, 2026

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes
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Pathogen Box screening for hit identification against Mycobacterium abscessus.

Jinsun Jeong1, Guehye Kim1, Cheol Moon2

  • 1Division of Applied Life Science (BK21plus Program), Gyeongsang National University, Jinju, South Korea.

Plos One
|April 27, 2018
PubMed
Summary

Researchers screened drug-like molecules to find new treatments for Mycobacterium abscessus infections. MMV688844 showed significant activity against this drug-resistant bacterium, offering a promising lead for future therapies.

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Area of Science:

  • Microbiology
  • Drug Discovery
  • Infectious Diseases

Background:

  • Mycobacterium abscessus is a rapidly growing, life-threatening bacterium known for its drug resistance.
  • There is a critical need for effective therapeutic regimens against M. abscessus infections.

Purpose of the Study:

  • To identify novel compounds active against Mycobacterium abscessus.
  • To screen the Pathogen Box library for potential M. abscessus drug candidates.

Main Methods:

  • Resazurin live/dead assays were employed for high-throughput screening.
  • A library of 400 drug-like molecules from the Pathogen Box was screened at 20 μM.
  • Hit compounds were further evaluated using dose-response curves to determine minimum inhibitory concentrations (MICs).

Main Results:

  • The initial screen identified four compounds exhibiting >80% growth inhibition.
  • Three hit candidates (MMV688508, MMV688844, MMV688845) were confirmed through dose-response analysis.
  • MMV688844 demonstrated the most potent activity, with excellent MIC values against wild-type M. abscessus and clinical isolates.

Conclusions:

  • MMV688844 is a promising candidate for further development in the M. abscessus drug discovery pipeline.
  • The Pathogen Box is a valuable resource for identifying novel antimicrobials against challenging pathogens.