Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Amyloid Fibrils03:03

Amyloid Fibrils

12.0K
Amyloid fibrils are aggregates of misfolded proteins.  Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils. 
Amyloid deposits were observed as early as 1639 in the liver and the spleen.   In 1854, Rudolph Virchow performed iodine staining,...
12.0K
Amyloid Fibrils03:03

Amyloid Fibrils

6.5K
6.5K
Yeast Signaling01:28

Yeast Signaling

17.3K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.3K
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

18.8K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
18.8K
Comparative Excretory Systems02:24

Comparative Excretory Systems

26.7K
Animals have evolved different strategies for excretion, the removal of waste from the body. Most waste must be dissolved in water to be excreted, so an animal’s excretory strategy directly affects its water balance.
26.7K
Comparing Experimental Results: Student's t-Test01:09

Comparing Experimental Results: Student's t-Test

6.1K
The t-test is a statistical method used to compare the sample mean with a population mean or compare two means from two data sets. The test statistic is calculated from the standard deviation, mean, and number of measurements in the data set at a selected confidence interval and then compared to a table of critical values at this confidence level. If the test statistic is smaller than the critical value, the null hypothesis is accepted. In this case, we state that the difference between the...
6.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Special Issue "Role of Immune Cells in Non-Infectious Inflammatory Diseases and Cancers".

International journal of molecular sciences·2026
Same author

Detection system- and strain-dependent diversity of de novo [PSI+] prion generation and phenotypes in Saccharomyces cerevisiae.

Journal of microbiology (Seoul, Korea)·2025
Same author

Linking SIRT1 Variants to Metabolic and Cardiovascular Phenotypes: Insights from Population Genetics and In Silico Structural Analysis.

Cell biochemistry and biophysics·2025
Same author

Shaping viral immunotherapy towards cancer-targeted immunological cell death.

Frontiers in oncology·2025
Same author

Yeast Prions: Discovery, Nature, Cellular Manipulation and Implication.

Journal of microbiology and biotechnology·2025
Same author

Anti-Prion Systems in Saccharomyces cerevisiae.

Journal of neurochemistry·2025

Related Experiment Video

Updated: Feb 11, 2026

Prion Safety Laboratory Swipe Test
06:01

Prion Safety Laboratory Swipe Test

Published on: February 14, 2025

1.3K

Yeast Prions Compared to Functional Prions and Amyloids.

Reed B Wickner1, Herman K Edskes1, Moonil Son1

  • 1Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda 20892-0830, MD, USA.

Journal of Molecular Biology
|April 27, 2018
PubMed
Summary

Yeast prions like [URE3] and [PSI+] are rare, detrimental amyloid proteins. They serve as models for human amyloid diseases, differing from functional amyloids.

Keywords:
Upf, Btn2p, Cur1p, Hsp104, Ssb1, Siw14amyloid of Sup35 or Ure2anti-prion systemsfolded in-register parallel beta sheetsinositol polyphosphates

More Related Videos

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
09:50

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Published on: September 29, 2017

14.4K
Author Spotlight: Insight Into Advances in Prion Diseases Research
10:40

Author Spotlight: Insight Into Advances in Prion Diseases Research

Published on: August 11, 2023

854

Related Experiment Videos

Last Updated: Feb 11, 2026

Prion Safety Laboratory Swipe Test
06:01

Prion Safety Laboratory Swipe Test

Published on: February 14, 2025

1.3K
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
09:50

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Published on: September 29, 2017

14.4K
Author Spotlight: Insight Into Advances in Prion Diseases Research
10:40

Author Spotlight: Insight Into Advances in Prion Diseases Research

Published on: August 11, 2023

854

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Saccharomyces cerevisiae harbors prions, which are misfolded proteins forming amyloid structures.
  • Key yeast prions include [URE3] (Ure2p) and [PSI+] (Sup35p), involved in nitrogen metabolism and translation termination, respectively.

Purpose of the Study:

  • To distinguish functional amyloids from detrimental yeast prions.
  • To highlight the characteristics of amyloid-based yeast prions and their relevance to human diseases.

Main Methods:

  • Comparative analysis of yeast prions ([URE3], [PSI+]) with functional amyloids ([Het-s], [BETA]).
  • Characterization of yeast prion properties: rarity, sporadic occurrence, variants, amyloid structure, host detriment, and curing mechanisms.

Main Results:

  • Amyloid-based yeast prions are rare, sporadic, and exhibit diverse variants.
  • They possess a specific in-register parallel β-sheet amyloid architecture.
  • These prions are harmful, induce stress responses, and can be eliminated by host systems.

Conclusions:

  • Yeast prions ([URE3], [PSI+]) are distinct from functional amyloids based on their characteristics.
  • These infectious yeast amyloidoses provide valuable models for studying human amyloid diseases with potential infectious links.