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Long noncoding RNA HOTTIP cooperates with CCCTC-binding factor to coordinate HOXA gene expression.

Feng Wang1, Zhongqiong Tang1, Honglian Shao2

  • 1Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 South Jingming Road, Chenggong District, Kunming, Yunnan Province, 650500, China.

Biochemical and Biophysical Research Communications
|April 27, 2018
PubMed
Summary

HOTTIP, a novel lincRNA, physically associates with CTCF to maintain HOXA gene expression and chromatin modifications. This reveals a functional link between lincRNAs and CTCF in gene activation and chromatin organization.

Keywords:
Chromatin boundaryHOX genesPositional identityTranscriptional regulationncRNAs

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Area of Science:

  • Genetics
  • Epigenetics
  • Molecular Biology

Background:

  • HOX gene spatiotemporal expression is crucial for development and relies on positional identity and genomic location.
  • The precise transcriptional and epigenetic regulation maintaining HOX expression patterns remains incompletely understood.

Purpose of the Study:

  • To investigate the role of HOTTIP, a long non-coding RNA, in the regulation of HOXA gene expression.
  • To elucidate the functional interaction between HOTTIP and CCCTC-binding factor (CTCF) in maintaining chromatin structure and gene activation.

Main Methods:

  • Investigated the physical association between HOTTIP and CTCF in human foreskin fibroblasts.
  • Analyzed the impact of this interaction on chromatin modifications and transcriptional activation of HOXA genes.

Main Results:

  • Demonstrated that HOTTIP physically associates with CTCF, a known insulator protein.
  • Showed that HOTTIP and CTCF cooperatively maintain chromatin modifications of HOXA genes.
  • Confirmed the coordination of transcriptional activation of distal HOXA genes by HOTTIP and CTCF.

Conclusions:

  • Revealed a functional connection between HOTTIP and CTCF in regulating HOXA gene expression.
  • Shed light on the role of long non-coding RNAs (lincRNAs) in gene activation.
  • Highlighted the involvement of CTCF-mediated chromatin organization in HOX gene regulation.