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Genotypic characterization of rotavirus in children under 5 years circulating in Côte D'Ivoire from 2010 to 2013

Catherine Boni-Cisse1,2, Sindou Meite3,4, Alice Britoh Mlan3,4

  • 1UFR des Sciences Médicales, Département de Microbiologie, Université Félix Houphouët Boigny, Abidjan, Côte d'Ivoire. bonicatc@yahoo.fr.

Virology Journal
|April 28, 2018
PubMed

Insights

Rotavirus surveillance in Côte d'Ivoire identified G12P[8] as the predominant strain before vaccine introduction. Continuous monitoring is recommended to guide vaccine choices and track genotypic variability.

Area of Science:

  • Virology
  • Epidemiology
  • Public Health

Background:

  • Rotavirus is a leading cause of severe gastroenteritis in young children globally.
  • The World Health Organization (WHO) recommends rotavirus strain surveillance prior to vaccine introduction.
  • Understanding pre-vaccine rotavirus epidemiology is crucial for public health strategies.

Purpose of the Study:

  • To characterize the epidemiology of rotavirus infections in children under five in Côte d'Ivoire.
  • To determine the circulating rotavirus genotypes before the introduction of vaccination programs.

Main Methods:

  • Enzyme Immunoassay (EIA) was used to detect rotavirus VP6 antigens in stool samples.
  • Reverse-transcriptase-PCR (RT-PCR) assays were employed for genotyping of positive samples.
  • Study included 684 children under five years, with informed parental consent.

Main Results:

  • Rotavirus VP6 antigens were detected in 27.1% of the tested samples.
  • The most common G genotypes were G12 (46.6%) and G1 (13.1%).
  • The predominant P genotype was P[8] (49.8%), with G12P[8] being the most prevalent strain (26.6%).

Conclusions:

  • The predominant rotavirus strain identified was G12P[8], informing vaccine selection.
  • Ongoing surveillance of rotavirus strains is essential due to observed genotypic variability.
  • Continuous monitoring post-vaccine introduction will aid in assessing vaccine effectiveness and adapting public health strategies.
Abstract

Keywords:
Côte d’IvoireGenotypeRotavirusSurveillance

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