Preservation of rat limbs by hyperbaric carbon monoxide and oxygen

Cold ischemia times ranging from <6 h to as long as 24 h are generally quoted as the limits for attempting the replantation of amputated extremities. In this study, we aimed to assess the effect of hyperbaric carbon monoxide (CO) and oxygen (O₂) on rat limb preservation. Donor rat limbs were preserved in a chamber filled with hyperbaric CO and O₂ for 3 days (CO + O₂ 3 days) or 7 days (CO + O₂ 7 days). Positive and negative control groups were created by using non-preserved limbs (NP) and limbs wrapped in saline-moistened gauze for 3 days (SMG 3 days), respectively. The survival rate of transplanted limbs at postoperative day 90 was 88% in the NP and 86% in the CO + O₂ 3 days. The corresponding survival rate was 50% in the CO + O₂ 7 days at postoperative day 90 but was 0% in the SMG 3 days at postoperative day 3. Muscle mass decreased in the CO + O₂ 3 days and CO + O₂ 7 days compared with the NP, but sciatic-tibial nerve conduction velocities did not differ. These results indicate that amputated extremities preservation with hyperbaric CO and O₂ could extend the time limits of preservation, maintaining their viability for replantation.