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Related Concept Videos

CRISPR01:59

CRISPR

58.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Genetic Screens02:46

Genetic Screens

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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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What is Genetic Engineering?00:49

What is Genetic Engineering?

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Overview
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Genetics of Speciation02:16

Genetics of Speciation

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Speciation is the evolutionary process resulting in the formation of new, distinct species—groups of reproductively isolated populations.
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Related Experiment Video

Updated: Feb 11, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

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[Functional genetic screening using CRISPR-Cas9 system].

Huanhuan Li1, Chenghao Huang1

  • 1National Institute of Diagnostics and Vaccine Development in Infectious Dciseases, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University, Xiamen 361102, Fujian, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|April 28, 2018
PubMed
Summary

Functional genetic screening aids biological research and drug discovery. The CRISPR-Cas9 system offers a powerful, efficient tool for high-throughput gene discovery and functional annotation in various research areas.

Keywords:
CRISPR/Cas9functional genetic screeninggenome-scale knockout library

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Area of Science:

  • Genetics
  • Molecular Biology
  • Bioinformatics

Background:

  • Functional genetic screening is crucial for understanding biological processes, disease development, and gene function.
  • Traditional methods face limitations in efficiency and throughput for large-scale genetic analysis.

Purpose of the Study:

  • To review the CRISPR-Cas9 system's characteristics.
  • To highlight the applications of CRISPR-Cas9 in functional genetic screening.

Main Methods:

  • Review of existing literature on CRISPR-Cas9 technology.
  • Analysis of CRISPR-Cas9 applications in various genetic screening contexts.

Main Results:

  • CRISPR-Cas9 enables precise and efficient genome editing.
  • The system facilitates high-throughput functional genetic screening for gene discovery.

Conclusions:

  • CRISPR-Cas9 is a transformative tool for genetic research.
  • Its application in functional screening accelerates the identification of genes critical for specific phenotypes and therapeutic targets.