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Photoelectrochemical platform for cancer cell glutathione detection based on polyaniline and nanoMoS2 composites
Xu Hun1, Yanxin Li1, Shiyu Wang1
1Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education; State Key Laboratory Base of Eco-chemical Engineering; Key Laboratory of Rubber-Plastics of Ministry of Education/Shandong Provincial Key Laboratory of Rubber Plastics; Key Laboratory of Biochemical Analysis, Shandong Province; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Abstract:
Herein, a visible light photoelectrochemical (PEC) platform based on polyaniline (PANI) and nanoMoS2 composites as optoelectronic material for glutathione detection without any auxiliary of biomolecules or labeled materials was developed. Firstly, the nanoMoS2 was prepared via a simple ultrasound exfoliation method. The PANI was synthesized by chemical oxidative polymerization method. Then composite of PANI and nanoMoS2 was used to modify gold electrode. It was found that the composite membrane showed excellent PEC properties. And glutathione enhanced the PEC signal greatly. Based on this finding a method for glutathione detection was fabricated. Under the optimum conditions, the linear response of glutathione concentrations ranged from 1.0 × 10-10 to 1.0 × 10-4 mol L-1 was obtained with a detection limit of 3.1 × 10-11 mol L-1. The relative standard deviation was 2.9% at 2.0 × 10-9 M (n = 10). This method showed high sensitivity and simpleness which opened up a new promising signal-on PEC platform for future bioassay.
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