Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Experimental Designs

Experimental Designs

An experimental design is a systematic process that allows researchers to evaluate the relationship between dependent and independent variables. There are three widely used types of experimental design - pre-experimental design, true experimental design, and quasi-experimental design. In pre-experimental design, the researcher compares the data before and after some interventions or treatments. The true-experimental design has more than one purposefully created group, a commonly measured...

Bioequivalence Experimental Study Designs: Completely Randomized and Randomized Block Designs

Bioequivalence Experimental Study Designs: Completely Randomized and Randomized Block Designs

Body:Bioequivalence experimental study designs are crucial methodologies used in evaluating and comparing the bioavailability of different drug products. These designs are categorized into various types: completely randomized, randomized block, repeated measures, cross and carry-over, and Latin square designs.Completely randomized designs involve randomly allocating treatments to all subjects participating in the experiment. This allocation is achieved by assigning unique random numbers to...

Reliability and Validity

Reliability and Validity

Reliability and validity are two important considerations that must be made with any type of data collection. Reliability refers to the ability to consistently produce a given result. In the context of psychological research, this would mean that any instruments or tools used to collect data do so in consistent, reproducible ways.

In Vitro Drug Release Testing: Overview, Development and Validation

In Vitro Drug Release Testing: Overview, Development and Validation

In vitro dissolution and drug release tests assess how quickly and how much of a drug is released from its dosage form into an aqueous medium under standardized laboratory conditions. These tests are essential tools in pharmaceutical development and quality assurance, offering insight into the drug's performance before clinical use.During formulation development, dissolution testing identifies incomplete or inconsistent drug release issues. It also supports decisions on selecting the optimal...

Group Design

Group Design

The most basic experimental design involves two groups: the experimental group and the control group. The two groups are designed to be the same except for one difference— experimental manipulation. The experimental group gets the experimental manipulation—that is, the treatment or variable being tested—and the control group does not. Since experimental manipulation is the only difference between the experimental and control groups, we can be sure that any differences between...

Development of Analytical Methods

Development of Analytical Methods

An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Insights into Selenium-Modulated Amino Acids and Carbohydrates as Osmolytes Linked to Photosynthetic Efficiency in Drought-Stressed Edamame.

Plants (Basel, Switzerland)·2026

Same author

Development & assessment of polyherbal extracts for treating oral cancer by integrating phytochemistry, bioactivities, and network pharmacology.

Scientific reports·2026

Same author

Traditional antidiabetic medicinal plants of Himachal Pradesh: ethnomedicinal evidence and drug-discovery potential.

Frontiers in nutrition·2026

Same author

Clinical Outcomes and Predictors of Response to Levodopa-Entacapone-Carbidopa Intestinal Gel in Advanced Parkinson's Disease: A Retrospective Cohort Study over a 4-Year Period.

Pharmaceutics·2026

Same author

Synthesis and characterization of Curcuma Caesia plant root extract-mediated ZnO nanoparticles: efficacy as soil conditioner and plant growth promoter.

Scientific reports·2026

Same author

Serum Dkk-1 Is Associated with Pain Intensity, Flare-Ups, and Bone Mineral Density in Non-Obese Patients with Knee Osteoarthritis: A Single-Center, Cross-Sectional Study.

International journal of molecular sciences·2026

Search research articles

Related Experiment Video

Updated: Feb 11, 2026

Validation of a Psychosocial Intervention on Body Image in Older People: An Experimental Design

Validation of a Psychosocial Intervention on Body Image in Older People: An Experimental Design

Published on: May 31, 2021

Reversed phase HPLC for strontium ranelate: Method development and validation applying experimental design.

Béla Kovács^1,2, Lajos Kristóf Kántor¹, Mircea Dumitru Croitoru³

¹Gedeon Richter Romania, 540306, Tîrgu Mureș, Romania.

Acta Pharmaceutica (Zagreb, Croatia)

|April 28, 2018

Summary

A validated reverse-phase HPLC method accurately quantifies strontium ranelate. This reliable analytical technique is suitable for routine quality control of strontium ranelate formulations.

Keywords:

HPLC experimental design full factorial design strontium ranelate

More Related Videos

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Published on: June 17, 2022

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography HPLC

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography HPLC

Published on: March 15, 2017

Related Experiment Videos

Last Updated: Feb 11, 2026

Validation of a Psychosocial Intervention on Body Image in Older People: An Experimental Design

Validation of a Psychosocial Intervention on Body Image in Older People: An Experimental Design

Published on: May 31, 2021

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Published on: June 17, 2022

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography HPLC

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography HPLC

Published on: March 15, 2017

Area of Science:

Analytical Chemistry
Pharmaceutical Analysis

Background:

Strontium ranelate is a key pharmaceutical agent.
Accurate quantification is essential for quality control.

Purpose of the Study:

To develop and validate a robust analytical method for strontium ranelate.
To ensure the method is suitable for routine pharmaceutical analysis.

Main Methods:

Reverse-phase High-Performance Liquid Chromatography (RP-HPLC).
Full factorial experimental design for method development.
Validation according to international guidelines (ICH).

Main Results:

Strontium ranelate eluted at 4.4 minutes with no excipient interference at 321 nm.
Method demonstrated excellent linearity (R2=0.99998) from 20-320 μg mL-1.
High accuracy (96.1-102.1% recovery) and precision (RSDs 1.0-1.4%) were confirmed.

Conclusions:

The developed RP-HPLC method is fast, reliable, and cost-effective.
The method is validated for linearity, selectivity, sensitivity, accuracy, and precision.
Proposed for routine analysis of strontium ranelate in pharmaceutical formulations.