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Updated: Feb 11, 2026

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
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Structure-Function Analysis of Chloroplast Proteins via Random Mutagenesis Using Error-Prone PCR.

Louis Dumas1, Francesca Zito2, Pascaline Auroy1

  • 1Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Commissariat à l'Energie Atomique, Centre National de la Recherche Scientifique, Aix-Marseille Université, Unité Mixte de Recherche 7265, BIAM, Commissariat à l'Energie Atomique Cadarache, 13115 Saint-Paul-lez-Durance, France.

Plant Physiology
|April 29, 2018
PubMed
Summary

Researchers developed a new method for creating random chloroplast gene mutants using error-prone PCR and complementation. This technique efficiently generates mutations for studying photosynthesis and directed evolution of chloroplast enzymes.

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