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Updated: Feb 11, 2026

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9
Published on: September 10, 2020
[Applications of the CRISPR/Cas9 system in insects]
Xiao Ling Tong1,2, Chun Yan Fang1,2, Tin Ting Gai1,2
1State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
Abstract:
The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated) system guides Cas9 to specific genomic locations by a short RNA search string. This technology enables the systematic interrogation of mammalian genome editing, repairing damaged genes, silencing harmful genes and improving quality traits. In recent years, with the introduction of the CRISPR/Cas9 system for easy, fast and efficient genetic modification, it has been possible to conduct meaningful functional studies in a broad array of insect species, such as Drosophila, Bombyx mori, Aedes aegypti and butterflies et al. In this review, we summarize the application of CRISPR/Cas9 in different insect species, discuss methods for its promotion, and consider its application for future insect studies.
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