Related Experiment Video

Updated: Feb 11, 2026

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
09:54

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

Published on: January 5, 2018

15.9K

Respiratory analysis of coupled mitochondria in cryopreserved liver biopsies

Mercedes García-Roche1, Alberto Casal2, Mariana Carriquiry2

  • 1Center for Free Radical and Biomedical Research (CEINBIO) and Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Uruguay; Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Uruguay.

Redox Biology
|April 29, 2018
PubMed

Keywords:
BiopsyCryopreservationHigh-resolution respirometryMitochondriaMitochondrial functionOxygen consumption rateliver biopsy cryopreservationmitochondrial respirometrycryoprotectant protocolstissue preservation methods

More Related Videos

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy11:27

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Published on: February 7, 2015

20.2K
Preparation of Highly Coupled Rat Heart Mitochondria12:28

Preparation of Highly Coupled Rat Heart Mitochondria

Published on: September 23, 2010

23.3K

Related Concept Videos

Peroxisomes and Mitochondria01:30

Peroxisomes and Mitochondria

Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
97.6K
Mitochondria01:37

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
20.5K
Spin–Spin Coupling: Two-Bond Coupling (Geminal Coupling)01:20

Spin–Spin Coupling: Two-Bond Coupling (Geminal Coupling)

Two NMR-active nuclei bonded to a central atom can be involved in geminal or two-bond coupling. Geminal coupling is commonly seen between diastereotopic protons in chiral molecules and unsymmetrical alkenes, among others.
The central atom need not be NMR-active because its electrons are affected by the electron polarization of the spin-active atoms. However, spin information is transmitted less effectively than in one-bond coupling, and 2J values are usually weaker than 1J values. The energy of...
1.7K
Spin–Spin Coupling: Three-Bond Coupling (Vicinal Coupling)01:22

Spin–Spin Coupling: Three-Bond Coupling (Vicinal Coupling)

Vicinal or three-bond coupling is commonly observed between protons attached to adjacent carbons. Here, nuclear spin information is primarily transferred via electron spin interactions between adjacent C‑H bond orbitals. This generally favors the antiparallel arrangement of spins, so 3J values are usually positive.
The extent of coupling depends on the C‑C bond length, the two H‑C‑C angles, any electron-withdrawing substituents, and the dihedral angle between the involved orbitals. The...
1.5K
Anatomy of Respiratory System II: Lower Respiratory Tract01:31

Anatomy of Respiratory System II: Lower Respiratory Tract

The lower respiratory tract is anatomically composed of several vital structures, including the larynx, trachea, bronchial tree, alveoli, lungs, and pleurae. Each component has a specific function, and all are intricately connected to ensure efficient respiration.
The Larynx
It is located between the pharynx and the trachea, acts as a passageway for air, and hosts several critical structures, such as the epiglottis, vocal cords, and glottis. The epiglottis acts as a gateway, guiding food to the...
3.9K
The Respiratory System01:16

The Respiratory System

The respiratory system is comprised of the organs that enable breathing. Air enters the nostrils and mouth, followed by the pharynx (throat) and larynx (voice box), which lead to the trachea (windpipe). In the thoracic cavity, the trachea splits into two bronchi that allow air to enter the lungs. The bronchi split into progressively smaller bronchioles and terminate in small groups of tiny sacs in the lungs called alveoli, where gas exchange occurs.
89.6K