Standardized methods for preparation and bi-variate length & diameter counting/sizing of aerosol and tissue digestion...

Most fiber length distributions fit a log-normal distribution with their being many more shorter fibers present as compared to the longer fibers. As the longer fibers have been suggested to be more important for possible pathogenesis giving equal weight to all fiber lengths when sizing fibers will under sample the longer fibers. The methods described here, are based upon the optimization of fiber counting/sizing rules over a number years of experience and have been developed to provide a stable estimate of the mean number of particles and fibers present in the size ranges: particles, fibers < 5 μm; 5-20 μm; and >20 μm. These methods were first applied using TEM, however, with the development of high resolution SEM, it was found that higher reproducibility could be obtained with SEM.