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Related Concept Videos

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Related Experiment Video

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A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility.

Hiroyuki Yamanaka1, Mitsuru Komeya1, Hiroko Nakamura2

  • 1Laboratory of Biopharmaceutical and Regenerative Sciences, Institute of Molecular Medicine and Life Science, Yokohama City University Association of Medical Science, Yokohama, Kanagawa 236-0004, Japan; Department of Urology, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa 236-0004, Japan.

Biochemical and Biophysical Research Communications
|April 30, 2018
PubMed
Summary
This summary is machine-generated.

Researchers developed a new microfluidic device (ML-D) that successfully maintains and induces mouse spermatogenesis for extended periods. This simpler device improves nutrient and oxygen supply, enabling detailed observation of sperm development.

Keywords:
AcrosomeMicrofluidic deviceOrgan cultureSpermatogenesisSpermiogenesis

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Area of Science:

  • Reproductive Biology
  • Biomedical Engineering
  • Microfluidics

Background:

  • Previous microfluidic devices (MFD) maintained mouse spermatogenesis for over 6 months.
  • Conventional culture methods have limitations in duration and observation.

Purpose of the Study:

  • To develop a simpler, more effective microfluidic device (ML-D) for inducing and maintaining spermatogenesis.
  • To improve the observation of morphological changes during spermiogenesis.
  • To establish a versatile platform for culturing various tissue types.

Main Methods:

  • Designed a novel monolayer device (ML-D) with a pillar and slit barrier structure.
  • Incorporated a new method for introducing sample tissue during device fabrication.
  • Utilized polydimethylsiloxane (PDMS) for nutrient and oxygen supply.
  • Employed a glass slide base for enhanced microscopic visibility.
  • Monitored Acr-Gfp transgenic mouse testis tissue daily.

Main Results:

  • The ML-D effectively induced and maintained mouse spermatogenesis for longer durations than conventional methods.
  • The device design facilitated nutrient and oxygen exchange, supporting tissue viability.
  • Daily imaging successfully recorded morphological changes of the acrosome during spermiogenesis.
  • The improved visibility allowed for detailed observation of cellular processes.

Conclusions:

  • The monolayer device (ML-D) is a simple and effective tool for inducing and maintaining spermatogenesis.
  • This technology offers enhanced visibility for observing dynamic biological processes in cultured tissues.
  • The adaptable sample loading method suggests potential applications beyond testis tissue culture.