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Updated: Feb 11, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
High performance liquid chromatography column efficiency enhancement by zero dead volume recycling and practical
Marek Minarik1, Martin Franc2, Milan Minarik2
1Watrex Praha s.r.o., Drnovska 1112/60, CZ - 161 00 Prague, Czech Republic; Department of Analytical Chemistry, Faculty of Sciences, Charles University, Albertov 6, CZ - 128 43 Prague, Czech Republic.
Abstract:
A new instrumental approach to recycling HPLC is described. The concept is based on fast reintroduction of incremental peak sections back onto the separation column. The re-circulation is performed within a closed loop containing only the column and two synchronized switching valves. By having HPLC pump out of the cycle, the method minimizes peak broadening due to dead volume. As a result the efficiency is dramatically increased allowing for the most demanding analytical applications. In addition, a parking loop is employed for temporary storage of analytes from the middle section of the separated mixture prior to their recycling.
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