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Modulation of Protein-Interaction States through the Cell Cycle
Lingyun Dai1, Tianyun Zhao1, Xavier Bisteau2
1School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore.
Cell
|May 1, 2018
Summary
This study reveals dynamic protein interactions throughout the cell cycle using proteome-wide cellular thermal shift assay (CETSA). It uncovers key protein complex modulations critical for cell cycle progression and establishes CETSA as a powerful tool.
Area of Science:
- Cell Biology
- Proteomics
- Biochemistry
Background:
- Cell cycle progression relies on intricate biochemical processes.
- Protein expression levels offer limited insight into cellular event regulation.
- Understanding dynamic protein interactions is crucial for cell cycle research.
Purpose of the Study:
- To investigate changes in protein interaction states across different cell cycle phases.
- To establish proteome-wide cellular thermal shift assay (CETSA) as a method for studying protein interactions in intact cells.
- To uncover novel molecular details of cell cycle regulation.
Main Methods:
- Proteome-wide implementation of the cellular thermal shift assay (CETSA).
- Analysis of protein interaction states during specific cell cycle phases.
- Global profiling of protein expression and interactions.
Main Results:
- Identified changes in interaction states for over 750 proteins during the cell cycle.
- Revealed modulation of numerous protein complexes in specific cell cycle phases (e.g., DNA replication, chromatin remodeling, transcription, translation, nuclear envelope disintegration).
- Observed minimal differences in interaction states between G1 and G2 phases, suggesting conserved biochemical processes.
Conclusions:
- Protein complex modulation is a key feature of cell cycle progression.
- Proteome-wide CETSA is a valuable strategy for studying dynamic protein interactions in vivo.
- The study provides novel molecular insights into cell cycle regulation and protein complex dynamics.
Keywords:
CETSAcell cyclecellular thermal shift assayprotein complexprotein interaction statequantitative proteomicsMore Related Videos
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