Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Variation?01:14

What is Variation?

18.6K
Apart from the measures of central tendency, distribution, outliers, and the changing characteristics of data with time, an important characteristic of any data set is its variation or spread. In some data sets, the data values are concentrated closely near the mean; in others, the data values are more widely spread out from the mean.
The range, standard deviation, standard error, and variance are the different measures of variation.
Range: The range is the difference between its maximum and...
18.6K
What is the Cell Cycle?00:56

What is the Cell Cycle?

10.6K
The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: the interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the...
10.6K
What is the Cell Cycle?01:04

What is the Cell Cycle?

243.6K
The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...
243.6K
RNA Stability01:53

RNA Stability

35.8K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.8K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.9K
Variation01:19

Variation

8.0K
An important characteristic of any set of data is the variation in the data. In some data sets, the data values are concentrated closely near the mean; in other data sets, the data values are more widely spread out from the mean. The most common measure of variation, or spread, is the standard deviation, which is the square root of variance.
When independent and dependent variables are plotted on a scatter plot, the slope of a line is a value that describes the rate of change between the two...
8.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identifying Novel Estrogenic Mitochondrial Targets in Hypothalamic Proopiomelanocortin Neurons by Chemoproteomics.

bioRxiv : the preprint server for biology·2026
Same author

METTL3 regulates exocytosis independently of m<sup>6</sup>A.

Science advances·2026
Same author

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026
Same author

mRAVE governs lysosomal catabolism through basal and mTORC1-regulated V-ATPase assembly.

The EMBO journal·2026
Same author

PAK1 activation drives divergent resistance mechanisms to aromatase inhibition and tamoxifen in a luminal: A breast cancer model.

Molecular oncology·2026
Same author

Mitochondrial dysfunction in muscle cells induced by snoring vibrations.

Mitochondrion·2026

Related Experiment Video

Updated: Feb 11, 2026

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
07:22

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

29.3K

Pervasive Protein Thermal Stability Variation during the Cell Cycle.

Isabelle Becher1, Amparo Andrés-Pons2, Natalie Romanov2

  • 1Genome Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.

Cell
|May 1, 2018
PubMed
Summary

This study reveals how protein stability and solubility change throughout the cell cycle, particularly during mitosis and G1. These biophysical changes reflect key cellular activities and remodeling events.

Keywords:
cell cycleproteomicsthermal proteome profiling

More Related Videos

Differential Scanning Calorimetry &#8212; A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
08:13

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Published on: March 4, 2017

40.4K
Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes
09:41

Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes

Published on: February 24, 2017

9.1K

Related Experiment Videos

Last Updated: Feb 11, 2026

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
07:22

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

29.3K
Differential Scanning Calorimetry &#8212; A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
08:13

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Published on: March 4, 2017

40.4K
Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes
09:41

Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes

Published on: February 24, 2017

9.1K

Area of Science:

  • Proteomics
  • Cell Biology
  • Biophysics

Background:

  • Quantitative mass spectrometry is crucial for understanding proteome-wide regulation.
  • Protein abundance and post-translational modifications are key to biological processes.

Purpose of the Study:

  • To systematically analyze proteome-wide protein thermal stability and solubility during the eukaryotic cell cycle.
  • To investigate the relationship between these biophysical properties and cellular functions.

Main Methods:

  • Quantitative mass spectrometry was employed to assess protein thermal stability and solubility.
  • Proteins were analyzed on a proteome-wide scale across different phases of the cell cycle.

Main Results:

  • Significant variations in protein thermal stability and solubility were observed, predominantly in mitosis and G1 phases.
  • Cell-cycle factors, polymerases, and chromatin remodelers showed varying thermal stability.
  • Protein thermal stability correlated with enzyme activity, DNA binding, and complex formation in situ.
  • Intrinsically disordered and mitotically phosphorylated proteins were stabilized and solubilized during mitosis.

Conclusions:

  • Protein biophysical properties undergo substantial remodeling during the cell cycle, especially in mitosis.
  • Mitotic remodeling involves stabilization and solubilization of specific protein classes, altering the cellular biophysical environment.
  • The findings provide a valuable resource for understanding proteome regulation during cell cycle transitions.