Related Experiment Video
Updated: Feb 11, 2026

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
Published on: October 17, 2016
In vivo preclinical verification of a multimodal diffuse reflectance and correlation spectroscopy system for sensing
Julia M Pakela1, Seung Yup Lee2, Taylor L Hedrick2
1Applied Physics Program, University of Michigan, Ann Arbor, MI 48109.
Abstract:
In reconstructive surgery, impeded blood flow in microvascular free flaps due to a compromise in arterial or venous patency secondary to blood clots or vessel spasms can rapidly result in flap failures. Thus, the ability to detect changes in microvascular free flaps is critical. In this paper, we report progress on in vivo pre-clinical testing of a compact, multimodal, fiber-based diffuse correlation and reflectance spectroscopy system designed to quantitatively monitor tissue perfusion in a porcine model's surgically-grafted free flap. We also describe the device's sensitivity to incremental blood flow changes and discuss the prospects for continuous perfusion monitoring in future clinical translational studies.
Related Concept Videos
The Sense of Self: Reflected Self-Appraisal and Social Comparison
Assessment of Diffusion and Perfusion
The Role of Diffusion in Respiration
Diffusion is the process by which molecules move from an area of higher concentration to an area of lower concentration. In the respiratory system, this...
Self-Evaluation: Self-Enhancement and Self-Verification
Correlation and Causation
Correlation versus Causation
If the dependent variable increases or decreases when the independent variable increases, there is a positive or negative...
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview
The ATR process begins by directing a beam...
2D NMR: Homonuclear Correlation Spectroscopy (COSY)

