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Characterization of miRNA and their target gene during chicken embryo skeletal muscle development

Endashaw Jebessa1,2, Hongjia Ouyang1,2, Bahareldin Ali Abdalla1,2

  • 1Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China.

Oncotarget
|May 1, 2018
PubMed

Insights

This study reveals key microRNAs (miRNAs) regulating chicken skeletal muscle development by analyzing gene and miRNA expression. Findings identify specific miRNAs involved in myoblast proliferation and muscle growth.

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genomics

Background:

  • MicroRNAs (miRNAs) are crucial regulators of gene expression, influencing cellular processes through mRNA degradation or translational inhibition.
  • Understanding the molecular mechanisms of skeletal muscle development is vital for addressing muscle-related disorders and improving livestock growth.

Purpose of the Study:

  • To investigate the roles of differentially expressed genes and miRNAs in chicken skeletal muscle development.
  • To construct miRNA-mRNA interaction networks and identify key regulatory pathways during embryonic and post-hatch stages.

Main Methods:

  • Comparative analysis of mRNA and miRNA expression profiles from chicken skeletal muscle at embryonic day E11, E16, and one day post-hatch (P1).
  • Construction of miRNA-mRNA interaction networks using target prediction and integration analysis of differentially expressed molecules.
  • Functional in vitro assays to validate miRNA-target gene interactions.

Main Results:

  • Integration analysis revealed that a small number of miRNAs regulate a large number of mRNAs.
  • Identified key biological processes including muscle maintenance, myoblast proliferation, sarcomere organization, and regulation of apoptosis.
  • Functional assays confirmed that miR-222a and miR-126-5p target CPEB3 and FGFR3, respectively, affecting mRNA levels.

Conclusions:

  • The study provides a comprehensive analysis of miRNA and mRNA expression during chicken skeletal muscle development.
  • Identified candidate miRNAs and their target genes crucial for regulating embryonic muscle development and growth.
  • These findings offer valuable insights for future research into miRNA-mediated regulation of muscle development.
Keywords:
chicken embryomiRNAmuscle

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