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Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
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Multispectral photoacoustic microscopy and optical coherence tomography using a single supercontinuum source.

M Bondu1,2, M J Marques1, P M Moselund2

  • 1University of Kent, Applied Optics Group, School of Physical Sciences, Ingram building, Canterbury CT2 7NH, United Kingdom.

Photoacoustics
|May 1, 2018
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Summary

A single supercontinuum (SC) source enables multimodal imaging, combining optical coherence tomography (OCT) and multispectral photoacoustic microscopy (MPAM). This versatile SC source offers deep tissue penetration and detailed imaging of biological samples.

Keywords:
Multimodal imagingMultispectral imagingOptical coherence tomographyPhotoacousticSupercontinuum

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Area of Science:

  • Biomedical Optics
  • Advanced Imaging Techniques

Background:

  • Multimodal imaging offers complementary information for biological sample analysis.
  • Supercontinuum (SC) sources provide broad spectral bandwidths suitable for various optical imaging modalities.

Purpose of the Study:

  • To demonstrate a single supercontinuum source for simultaneous optical coherence tomography (OCT) and multispectral photoacoustic microscopy (MPAM).
  • To showcase the capabilities of a versatile SC source for multimodal biomedical imaging.

Main Methods:

  • Utilized a 2-octave bandwidth (475-2300 nm) supercontinuum source.
  • Selected the IR band (1310 nm) for OCT and the visible band (500-840 nm) for MPAM.
  • Achieved >35 nJ energy within <50 nm bandwidth for wavelengths >500 nm.

Main Results:

  • Demonstrated successful multimodal imaging using a single SC source.
  • Acquired in vitro B-scan images of mouse ear tissue.
  • Showcased the SC source's suitability for both OCT and MPAM applications.

Conclusions:

  • A single SC source can effectively drive multimodal imaging systems.
  • This approach simplifies instrumentation while maintaining imaging performance.
  • The demonstrated system holds potential for advanced biological tissue analysis.