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Microsatellite genotyping of clinical Candida parapsilosis isolates

Hamid Badali1, Sassan Rezaie2, Jacques F Meis3,4

  • 1Department of Medical Mycology/Invasive Fungi Research Center (IFRC), School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

Current Medical Mycology
|May 1, 2018
PubMed
Abstract

Insights

Molecular epidemiology of Candida parapsilosis in Iran reveals high genetic diversity among clinical isolates using microsatellite genotyping. This technique is valuable for tracking outbreaks of this common nosocomial pathogen.

Area of Science:

  • Medical Mycology
  • Molecular Epidemiology
  • Infectious Diseases

Background:

  • Candida parapsilosis is a frequent cause of hospital-acquired infections.
  • Limited data exists on the molecular epidemiology of clinical C. parapsilosis strains.

Purpose of the Study:

  • To investigate the microsatellite genotyping of clinical Candida parapsilosis isolates in Iran.
  • To assess the genetic diversity of C. parapsilosis in a clinical setting.

Main Methods:

  • Genotyping of 81 independent clinical C. parapsilosis isolates.
  • Utilized a panel of six microsatellite markers for short tandem repeat (STR) typing.

Main Results:

  • Identified 68 distinct genotypes among the isolates.
  • Observed high genetic diversity with a Simpson's diversity index of 0.9951.
  • Found heterogeneity between Iranian and Netherlands clinical isolates.

Conclusions:

  • Clinical C. parapsilosis isolates in Iran exhibit significant genetic diversity.
  • Microsatellite genotyping is a useful tool for screening and outbreak investigations involving C. parapsilosis.
Keywords:
Candida parapsilosisGenotypingIranMicrosatellite

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