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Published on: July 4, 2017
Effects of growth hormone and functional appliance on mandibular growth in an adolescent rat model
Objectives:
To investigate the individual and synergistic effects of growth hormone (GH) and functional appliance (FA) on mandibular growth in an adolescent rat model.
Materials And Methods:
Forty adolescent (6-week-old) female Wistar rats were randomly divided into four groups (10 rats in each group). The control group received a sham treatment (intra-abdominal injection of phosphate-buffered saline), the GH group received an intra-abdominal injection of recombinant human growth hormone, the FA group was treated with a mandibular advancement device, and the GH+FA group received both the GH and FA treatments. The amount of mandibular growth in each group was measured quantitatively using cone-bean computed tomography. The growth of condylar cartilage and expression of matrix metalloproteinases-1 and -13 (MMP-1 and MMP-13) and type II and X collagen (Col II and Col X) were assessed using histological staining and immunostaining techniques.
Results:
After 4 weeks, there was significant mandibular growth in the FA group compared with the control group ( P < .05). The GH+FA group had significantly greater mandibular length, thickness of condylar cartilage, and expression of MMP-1, MMP-13, Col II, and Col X in the cartilage than the other groups ( P < .05). The GH+FA group and GH group had significantly greater weight than the FA and control groups ( P < .05).
Conclusions:
The FA as well as GH+FA stimulated mandibular growth in adolescent rats.
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