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Updated: Feb 11, 2026

Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature
Published on: October 14, 2022
Effects of ex vivo aging and storage temperature on blood viscosity
Jeffrey S Horner1, Antony N Beris1, Donna S Woulfe2
1Center for Molecular and Engineering Thermodynamics and Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, USA.
Background:
Research on hemorheology is driven in part by its significance in blood diseases and the possible use of hemorheology as a diagnostic tool. However, existing data on blood rheology are limited largely to measurements of steady shear behavior often with varying measurement protocols and insufficient characterization of the physiology.
Objective:
The effects of ex vivo aging and environmental conditions on blood viscosity are investigated to improve standards for hemorheology measurements.
Methods:
Measurements on the viscosity of blood from nine healthy donors are obtained and the physiological state of the blood determined. Steady and transient shear measurements are reported as a function of time from withdrawal. The effect of transportation temperature is also assessed.
Results:
Blood transported at 4 °C may exhibit anomalous viscosity variations for short to intermediate times, as opposed to blood transported at room temperature. A time of approximately 3.0 hours was identified as the maximum time after the initial test that accurate rheological tests can be conducted on blood samples.
Conclusions:
Measurement protocol and time limit guidelines are established for conducting accurate rheological measurements on blood.
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