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Updated: Feb 11, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

An Efficient Venus Flytrap for the Reversible Binding of Nitric Oxide.

Rajendra Rathore¹, Sergey V Lindeman¹, Jay K Kochi²

¹(without address).

Angewandte Chemie (International Ed. in English)

|May 2, 2018

Summary

A novel stilbenoid derivative 1 demonstrates highly efficient, reversible binding of nitric oxide (NO). This interaction is controllable via simple oxidation and reduction processes.

Keywords:

Host-guest chemistry Molecular tweezers Nitric oxide Noncovalent interactions

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Area of Science:

Chemical biology
Molecular interactions
Organic chemistry

Background:

Nitric oxide (NO) plays crucial roles in biological systems.
Developing efficient methods for NO detection and control is of significant interest.
Stilbenoid derivatives offer versatile chemical scaffolds.

Purpose of the Study:

To investigate the binding characteristics of a specific stilbenoid derivative (compound 1) with nitric oxide (NO).
To explore the controllability of the NO-stilbenoid interaction through redox mechanisms.

Main Methods:

Synthesis and characterization of stilbenoid derivative 1.
Binding studies to quantify the interaction with nitric oxide.
Redox titration experiments to assess control over the binding.

Main Results:

Compound 1 exhibits remarkably efficient reversible binding of nitric oxide (NO).
The binding affinity is high, with a dissociation constant (K) greater than 3×10^6 M^-1.
The noncovalent interaction between compound 1 and NO is readily modulated by simple oxidation and reduction.

Conclusions:

Stilbenoid derivative 1 represents a promising molecule for NO sensing or sequestration.
The facile redox control over NO binding offers potential for dynamic molecular systems.
This study highlights the utility of tailored organic molecules in managing reactive species like NO.