Related Experiment Videos
Transmembrane Transport of Adenosine 5'-Triphosphate Using a Lipophilic Cholesteryl Derivative
Annett Kreimeyer1, François André2, Catherine Gouyette1
1Unité de Chimie Organique, URA CNRS 487, Département de BGM, Institut Pasteur, 28, rue du Docteur Roux, F-75724 Paris Cedex 15 (France), Fax: (+33) 1-45-68-84-04.
Angewandte Chemie (International Ed. in English)
|May 2, 2018
Abstract:
The uptake of ATP in liposomes was achieved by using the lipophilic derivative cholesteryloxycarbonyl-ATP (1). Its hydrolysis leading to the release of ATP inside the vesicules (see scheme) was observed with the help of a pH gradient and monitored by 31 P NMR spectroscopy. This is the first successful transfer of a nucleoside 5'-triphosphate across a membrane.