ESI Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (ESI-FT-ICR-MS): A Rapid High-Resolution Analytical
Tilmann B Walk1, Axel W Trautwein1, Hartmut Richter1
1Institut für Organische Chemie der Universität, Auf der Morgenstelle 18, D-72076 Tübingen (Germany), Fax: (+49) 7071-29-5560.
Angewandte Chemie (International Ed. in English)
|May 2, 2018
Summary
Direct elemental composition analysis of combinatorial chemistry compounds is now possible using electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR MS). Coupling with HPLC enhances high-resolution analytical capabilities for complex mixtures.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Organic Chemistry
Background:
- Combinatorial chemistry enables the rapid synthesis of large compound libraries.
- Accurate elemental composition determination is crucial for characterizing synthesized compounds.
- Existing mass spectrometry techniques may lack the resolution for complex mixtures.
Purpose of the Study:
- To demonstrate the direct elemental composition determination of combinatorial chemistry compounds.
- To showcase the benefits of coupling High-Performance Liquid Chromatography (HPLC) with ESI-FT-ICR MS.
- To highlight the superior resolution of ESI-FT-ICR MS over quadrupole mass spectrometry.
Main Methods:
- Electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR MS) was employed.
- High-Performance Liquid Chromatography (HPLC) was coupled with ESI-FT-ICR MS.
- Analysis of compounds from solid-phase synthesis was performed.
Main Results:
- Direct elemental composition determination of compound collections was achieved.
- The combination of HPLC and ESI-FT-ICR MS provided high-resolution, informative analysis.
- ESI-FT-ICR MS demonstrated improved resolution compared to quadrupole mass spectrometry for a solid-phase synthesized compound.
Conclusions:
- ESI-FT-ICR MS is a powerful tool for direct elemental composition analysis in combinatorial chemistry.
- HPLC coupling significantly enhances the analytical power of ESI-FT-ICR MS for complex samples.
- This technique offers superior resolution for characterizing synthesized compounds.
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