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Dual Recognition of Double-Stranded DNA by 2'-Aminoethoxy-Modified Oligonucleotides
Bernard Cuenoud1, Florence Casset2, Dieter Hüsken2
1Novartis Horsham Research Centre, Wimblehurst road, Horsham, West Sussex, RH12 4AB (UK), Fax: (+44) 1403-32 33 07.
Angewandte Chemie (International Ed. in English)
|May 2, 2018
Abstract:
Simultaneous interaction of the 2'-aminoethoxy-modified oligonucleotides with the phosphodiester backbone (shown on the right, A) and with the bases through Hoogsteen base contacts (B) is seen at each base-pair step of the duplex DNA target. The electrostatic interaction between the protonated amino group and the negatively charged phosphate group provides for a dramatic increase in the binding affinity and the association rate constant.