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Updated: Feb 11, 2026

Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines
Published on: November 25, 2017
Synthesis of Immobilized CMP-Sialic Acids and Their Enzymatic Transfer with Sialyltransferase
Yasuhiro Kajihara1, Takashi Ebata2, Hisashi Kodama2
1Department of System Function, Faculty of Science, Yokohama City University, 22-2, Seto, Kanazawa-ku, Yokohama, 236-0027 (Japan), Fax: (+81) 45-787-2370.
Abstract:
A novel immobilization procedure for glycoproteins is based on the reacion of immobilized CMP-NeuAc (CMP-sialic acids) with sialyltransferase (see scheme). The transfer ability was tested under various conditions; a corresponding oligosaccharide or asialoglycoprotein acted as sialyl acceptor. The solid-phase synthesis of CMP-NeuAc is also described.
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