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Large Circular Dichroism Ellipticities for N-Templated Helical Polypeptides Are Inconsistent with Currently Accepted
Peter Wallimann1, Robert J Kennedy1, Daniel S Kemp1
1Department of Chemistry, Rm. 18-582, Massachusetts Institute of Technology, Cambridge, MA 02139 (USA) Fax: (+1) 617-258-7500.
Abstract:
An unprecedented, high degree of helicity as judged by CD spectroscopy is observed in N-templated model peptides of the type AcHel-(Ala4 Lys)n Ala2 -NH2 (AcHel-Ala peptide pictured; AcHel is an N-terminal helix-inducing template for polypeptides). These results raise concern over the current methods for determining 100 % helicity.
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