Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

4.0K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
4.0K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

9.1K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
9.1K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

9.4K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
9.4K
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

3.1K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
3.1K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

4.7K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
4.7K
COP Coated Vesicles00:59

COP Coated Vesicles

17.4K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
17.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bacterial community associated with the surface and inside of centipede forcipules: Identification and characterization.

PloS one·2026
Same author

Response to "Critical Appraisal on Hydrogen Inhalation Therapy Alone May Not Alleviate Intestinal Mucosal Damage in NOMI Without Total-Layer Necrosis: An Experimental Swine Model Study".

Acute medicine & surgery·2025
Same author

Long-Term Continuous Heart Rate Variability Monitoring Over 20 Days for Severe Tetanus: A Case Report.

The American journal of case reports·2025
Same author

Hydrogen inhalation therapy alone may not alleviate intestinal mucosal damage in NOMI without total-layer necrosis: An experimental swine model study.

Acute medicine & surgery·2025
Same author

Continuous renal replacement therapy with cytokine-adsorbing hemofilter to control resuscitative endovascular balloon occlusion of the aorta-related ischemia-reperfusion injury in a swine hemorrhagic shock model.

European journal of trauma and emergency surgery : official publication of the European Trauma Society·2025
Same author

Establishment of a swine experimental model of non-occlusive mesenteric ischemia: Combining induced hemorrhagic shock and vasopressor administration.

Acute medicine & surgery·2024

Related Experiment Video

Updated: Jan 18, 2026

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
10:43

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Published on: July 19, 2022

2.9K

Selective Vesicle Formation from Calixarenes by Self-Assembly.

Yasutaka Tanaka1, Masami Miyachi2, Yoshiaki Kobuke2

  • 1PRESTO Japan Science and Technology Corporation, and Department of Materials Science, Shizuoka University, Hamamatsu, Shizuoka 432-8561 (Japan), Fax: (+81) 53-478-1199.

Angewandte Chemie (International Ed. in English)
|May 2, 2018
PubMed
Summary

Amphiphilic polyhydroxy macrocycles self-assemble into uniform, stable vesicles in water. Their selective aggregation is linked to macrocyclic structure and hydrogen bonding of phenolic groups.

Keywords:
CalixarenesHydrogen bondsSelf-assemblyVesicles

More Related Videos

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

6.3K
In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth
07:10

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

Published on: June 28, 2019

6.1K

Related Experiment Videos

Last Updated: Jan 18, 2026

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
10:43

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Published on: July 19, 2022

2.9K
Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

6.3K
In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth
07:10

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

Published on: June 28, 2019

6.1K

Area of Science:

  • Supramolecular chemistry
  • Materials science

Background:

  • Amphiphilic molecules can self-assemble into various structures.
  • Polyhydroxy macrocycles offer unique structural features for self-assembly.

Purpose of the Study:

  • To investigate the self-assembly behavior of amphiphilic polyhydroxy macrocycles in water.
  • To understand the factors influencing vesicle formation and stability.

Main Methods:

  • Synthesis of amphiphilic polyhydroxy macrocycles.
  • Vesicle formation studies in aqueous solutions.
  • Characterization of vesicle size and stability.

Main Results:

  • Selective formation of uniform vesicles (50-200 nm) from amphiphilic polyhydroxy macrocycles.
  • Vesicles exhibit stability in both aqueous solutions and dried forms.
  • Aggregation selectivity correlates with macrocyclic structure and phenolic hydrogen bonding.

Conclusions:

  • Amphiphilic polyhydroxy macrocycles are effective building blocks for vesicle formation.
  • Structural features, including macrocyclic moiety and hydrogen bonding, dictate self-assembly.
  • These vesicles show potential for various applications due to their stability and controlled formation.