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Updated: Feb 11, 2026

Imaging Protein-protein Interactions in vivo
Published on: October 10, 2010
In Vivo Protein-Protein Interaction Assays: Beyond Proteins
Hening Lin1, Virginia W Cornish1
1Department of Chemistry Columbia University New York, NY 10027 (USA) Fax: (+1) 212-932-1289.
Abstract:
The scope of cell-based assays is being expanded to allow the detection of interactions between proteins and DNA, RNA, or small molecules. The yeast two-hybrid assay, which normally detects protein-protein interactions, can be modified to detect interactions between ligands (the two dark yellow shapes in the diagram) and receptors (DHFR or GR). A small bridging molecule (made up of the ligands joined together) is used to join two fusion proteins (DHFR-DBD and AD-GR), thereby activating the reporter gene.
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