Highly Sensitive Novel Biosensor Based on an Immobilized lac Repressor

Area of Science:

• Biotechnology
• Biosensor Development
• Molecular Biology

Background:

• The lac repressor protein undergoes conformational changes upon binding to inducer molecules.
• These changes present an opportunity for developing sensitive detection systems.

Purpose of the Study:

• To develop a biosensor utilizing the lac repressor's conformational change.
• To enable detection of specific inducer molecules or operator sequences.

Main Methods:

• Covalent immobilization of the lac repressor protein onto a gold electrode.
• Utilizing a capacitive signal transducer to detect conformational alterations.
• Translating protein conformational changes into measurable electrical signals.

Main Results:

• Demonstrated the ability to detect specific inducer molecules via the biosensor.
• Showcased the potential for detecting corresponding operator sequences.
• Successfully translated protein conformational changes into a quantifiable signal.

Conclusions:

• The developed biosensor offers a sensitive method for detecting specific molecules.
• Lac repressor conformational changes can be effectively harnessed for biosensing applications.
• This approach provides a foundation for novel molecular detection technologies.