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Prokaryotic Transcriptional Activators and Repressors01:58

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The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
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The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...
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Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by...
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Highly Sensitive Novel Biosensor Based on an Immobilized lac Repressor.

Ibolya Bontidean1, Ashok Kumar1, Elisabeth Csöregi1

  • 1Department of Biotechnology Center for Chemistry and Chemical Engineering Lund University 22100, Lund (Sweden) Fax: (+46) 46-222-4713.

Angewandte Chemie (International Ed. in English)
|May 2, 2018
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Summary

A novel biosensor detects specific molecules by measuring conformational changes in the lac repressor protein. This sensitive repressor-based system translates molecular binding into a measurable electrical signal.

Keywords:
DNA recognitionbiosensorscapacitance measurementproteins

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Area of Science:

  • Biotechnology
  • Biosensor Development
  • Molecular Biology

Background:

  • The lac repressor protein undergoes conformational changes upon binding to inducer molecules.
  • These changes present an opportunity for developing sensitive detection systems.

Purpose of the Study:

  • To develop a biosensor utilizing the lac repressor's conformational change.
  • To enable detection of specific inducer molecules or operator sequences.

Main Methods:

  • Covalent immobilization of the lac repressor protein onto a gold electrode.
  • Utilizing a capacitive signal transducer to detect conformational alterations.
  • Translating protein conformational changes into measurable electrical signals.

Main Results:

  • Demonstrated the ability to detect specific inducer molecules via the biosensor.
  • Showcased the potential for detecting corresponding operator sequences.
  • Successfully translated protein conformational changes into a quantifiable signal.

Conclusions:

  • The developed biosensor offers a sensitive method for detecting specific molecules.
  • Lac repressor conformational changes can be effectively harnessed for biosensing applications.
  • This approach provides a foundation for novel molecular detection technologies.